Regeneration, morphological and molecular characterization

 

REGENERATION

Regeneration is performed when viability is below of 85% or the amount of seeds is not enough to respond to requests. Regeneration is carried out according to the breeding system of each species in order to use the appropriated isolation systems. The number of plants used for regeneration depends on the autogamy or allogamy reproductive system. The methodology used for regeneration of the most important crops is given in the following table.

Crop

Breeding system

Pollination mechanism

Isolation

Number of plants

Onion and leek

Allogamy

Insects

Mesh tunnel

30

Brassicas

Allogamy

Insects

Túnel de malla

30

Tomato

Autogamia

Selfing

--

7

Tomato wild relatives

Allogamy y autogamy

Manual pollination or insects

Cultivation in insect-proof greenhouse 

10

Pepper

Partial allogamy 

Selfing

Cultivation in insect-proof greenhouse 

10

Eggplant

Autogamy

Selfing

--

10

Lettuce

Autogamy

Selfing

--

10

Melon, cucumber, pumpkin and watermelon

Allogamy

Manual pollination or insects

Mesh tunnel or greenhouses

10

Bean, pea

Autogamy

Selfing

--

10

Spinach

Allogamy

Wind

Isolation by distance

30

 


 

MORPHOLOGICAL CHARACTERIZATION

Characterization is conducted using the descriptors edited by Bioversity International. Minimum descriptor lists developed by the ECPGR Crop Working Groups are always recorded. Some specific descriptors developed by the Genebank are used in some cases.


Traditional spanish tomato varieties

 


Spanish types of squash

 

MOLECULAR CHARACTERIZATION

The Genebank belongs to COMAV Institute in which several research lines are developed, some of them using the new molecular and genomic technologies. Molecular markers have been used to: - molecular variability studies and genetic diversity estimation –geographical distribution of the variation and its implication on conservation strategies – phylogenetic studies – molecular characterization of new species – core collections construction.

RAPD, AFLP, SSR and SRAP were used in the first studies. More recently SNP genotyping and sequencing of specific genes are performed. The species in which these studies are conducted are tomato, pepper, eggplant and wild relatives of these crops, melon, Cucurbita pepo, C. máxima, C. moschata and C. ficifolia.